Products

Human ASAH1(Acid ceramidase) ELISA Kit

EH6481 Catalogue No.
Acid ceramidase ELISA Kit, AC ELISA Kit, ACDase ELISA Kit, Acid Cdase ELISA Kit, Acylsphingosine deacylase ELISA Kit, N-acylethanolamine hydrolase ASAH1 ELISA Kit, N-acylsphingosine amidohydrolase ELISA Kit, Putative 32 kDa heart protein ELISA Kit, PHP32 ELISA Kit, Acid ceramidase subunit alpha ELISA Kit, Acid ceramidase subunit beta ELISA Kit, ASAH ELISA Kit, HSD-33 ELISA Kit, HSD33 ELISA Kit, ASAH1 ELISA Kit
Reactivity:
Human
Range:
0.156-10ng/ml
Sensitivity:
0.094ng/ml
Product Name
Human ASAH1(Acid ceramidase) ELISA Kit
Catalogue No.
EH6481
Alias
Acid ceramidase ELISA Kit, AC ELISA Kit, ACDase ELISA Kit, Acid Cdase ELISA Kit, Acylsphingosine deacylase ELISA Kit, N-acylethanolamine hydrolase ASAH1 ELISA Kit, N-acylsphingosine amidohydrolase ELISA Kit, Putative 32 kDa heart protein ELISA Kit, PHP32 ELISA Kit, Acid ceramidase subunit alpha ELISA Kit, Acid ceramidase subunit beta ELISA Kit, ASAH ELISA Kit, HSD-33 ELISA Kit, HSD33 ELISA Kit, ASAH1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
 ASAH1 ELISA Kit allows for the in vitro quantitative determination of ASAH1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Q13510
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of ASAH1 and the recovery rates were calculated by comparing the measured value to the expected amount of ASAH1 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10593
EDTA plasma(n=5)85-10491
heparin plasma(n=5)86-9792
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASAH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-105%89-105%88-100%
EDTA plasma(n=5)82-95%93-100%82-93%
heparin plasma(n=5)80-98%83-98%82-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only