- Product Name
- Human ASAH1(Acid ceramidase) ELISA Kit
- Catalogue No.
- EH6481
- Alias
- Acid ceramidase ELISA Kit, AC ELISA Kit, ACDase ELISA Kit, Acid Cdase ELISA Kit, Acylsphingosine deacylase ELISA Kit, N-acylethanolamine hydrolase ASAH1 ELISA Kit, N-acylsphingosine amidohydrolase ELISA Kit, Putative 32 kDa heart protein ELISA Kit, PHP32 ELISA Kit, Acid ceramidase subunit alpha ELISA Kit, Acid ceramidase subunit beta ELISA Kit, ASAH ELISA Kit, HSD-33 ELISA Kit, HSD33 ELISA Kit, ASAH1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ASAH1 ELISA Kit allows for the in vitro quantitative determination of ASAH1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- UniProt ID
- Q13510
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ASAH1 and the recovery rates were calculated by comparing the measured value to the expected amount of ASAH1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-105 | 93 |
EDTA plasma(n=5) | 85-104 | 91 |
heparin plasma(n=5) | 86-97 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASAH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-105% | 89-105% | 88-100% |
EDTA plasma(n=5) | 82-95% | 93-100% | 82-93% |
heparin plasma(n=5) | 80-98% | 83-98% | 82-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only