- Product Name
- Human HIST1H2BB(Histone H2B type 1-B) ELISA Kit
- Catalogue No.
- EH4119
- Alias
- HIST1H2BB ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- HIST1H2BB ELISA Kit allows for the in vitro quantitative determination of HIST1H2BB concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- UniProt ID
- P33778
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of HIST1H2BB and the recovery rates were calculated by comparing the measured value to the expected amount of HIST1H2BB in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-99 | 92 |
EDTA plasma(n=5) | 91-99 | 95 |
heparin plasma(n=5) | 85-104 | 99 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIST1H2BB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 92-102% | 93-104% | 85-105% |
EDTA plasma(n=5) | 82-100% | 88-101% | 85-96% |
heparin plasma(n=5) | 81-94% | 82-97% | 82-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only