Products

Human anti-GBM(Anti glomerular basement membrane antibody) ELISA Kit

EH4078 Catalogue No.
anti-GBM ELISA Kit, Anti glomerular basement membrane antibody ELISA Kit
Reactivity:
Human
Range:
1.563-100ng/ml
Sensitivity:
0.938ng/ml
Product Name
Human anti-GBM(Anti glomerular basement membrane antibody) ELISA Kit
Catalogue No.
EH4078
Alias
anti-GBM ELISA Kit, Anti glomerular basement membrane antibody ELISA Kit
Detection Method
Sandwich ELISA, Double Antigen
Application
 anti-GBM ELISA Kit allows for the in vitro quantitative determination of anti-GBM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of anti-GBM and the recovery rates were calculated by comparing the measured value to the expected amount of anti-GBM in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)95-10599
EDTA plasma(n=5)97-10599
heparin plasma(n=5)97-10399
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-GBM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)90-96%97-102%97-104%
EDTA plasma(n=5)89-99%89-101%89-100%
heparin plasma(n=5)90-96%90-96%90-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only