- Product Name
- Human anti-GBM(Anti glomerular basement membrane antibody) ELISA Kit
- Catalogue No.
- EH4078
- Alias
- anti-GBM ELISA Kit, Anti glomerular basement membrane antibody ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antigen
- Application
- anti-GBM ELISA Kit allows for the in vitro quantitative determination of anti-GBM concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of anti-GBM and the recovery rates were calculated by comparing the measured value to the expected amount of anti-GBM in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 95-105 | 99 |
EDTA plasma(n=5) | 97-105 | 99 |
heparin plasma(n=5) | 97-103 | 99 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-GBM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 90-96% | 97-102% | 97-104% |
EDTA plasma(n=5) | 89-99% | 89-101% | 89-100% |
heparin plasma(n=5) | 90-96% | 90-96% | 90-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only