- Product Name
- Human β-actin(Beta actin) ELISA Kit
- Catalogue No.
- EH4017
- Alias
- β-actin ELISA Kit, ACTB ELISA Kit, actin ELISA Kit, beta ELISA Kit, actin ELISA Kit, cytoplasmic 1 ELISA Kit, beta cytoskeletal actin ELISA Kit, Beta-actin ELISA Kit, PS1TP5-binding protein 1 ELISA Kit, PS1TP5BP1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- β-actin ELISA Kit allows for the in vitro quantitative determination of β-actin concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- P60709
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of β-actin and the recovery rates were calculated by comparing the measured value to the expected amount of β-actin in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-103 | 93 |
EDTA plasma(n=5) | 85-97 | 91 |
heparin plasma(n=5) | 85-103 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-actin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-97% | 98-104% | 85-102% |
EDTA plasma(n=5) | 96-100% | 85-94% | 85-98% |
heparin plasma(n=5) | 80-100% | 81-99% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only