- Product Name
- Human PEA(Pseudomonas Exotoxin A) ELISA Kit
- Catalogue No.
- EH4007
- Alias
- PEA ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PEA ELISA Kit allows for the in vitro quantitative determination of PEA concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- UniProt ID
- P11439
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PEA and the recovery rates were calculated by comparing the measured value to the expected amount of PEA in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-104 | 95 |
EDTA plasma(n=5) | 85-104 | 95 |
heparin plasma(n=5) | 86-103 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 97-102% | 89-98% | 86-97% |
EDTA plasma(n=5) | 86-100% | 84-101% | 82-97% |
heparin plasma(n=5) | 80-99% | 84-98% | 80-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only