Products

Human CHAT(Choline O-acetyltransferase) ELISA Kit

EH4004 Catalogue No.
ChAT ELISA Kit, CHOACTase ELISA Kit, Choline acetylase ELISA Kit, Choline O-acetyltransferase ELISA Kit
Reactivity:
Human
Range:
15.625-1000pg/ml
Sensitivity:
9.375pg/ml
Product Name
Human CHAT(Choline O-acetyltransferase) ELISA Kit
Catalogue No.
EH4004
Alias
ChAT ELISA Kit, CHOACTase ELISA Kit, Choline acetylase ELISA Kit, Choline O-acetyltransferase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CHAT ELISA Kit allows for the in vitro quantitative determination of CHAT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
P28329
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of CHAT and the recovery rates were calculated by comparing the measured value to the expected amount of CHAT in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10496
EDTA plasma(n=5)87-10595
heparin plasma(n=5)91-10397
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)96-103%86-101%88-100%
EDTA plasma(n=5)85-97%82-100%82-100%
heparin plasma(n=5)85-99%83-98%80-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only