- Product Name
- Human CHAT(Choline O-acetyltransferase) ELISA Kit
- Catalogue No.
- EH4004
- Alias
- ChAT ELISA Kit, CHOACTase ELISA Kit, Choline acetylase ELISA Kit, Choline O-acetyltransferase ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CHAT ELISA Kit allows for the in vitro quantitative determination of CHAT concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- UniProt ID
- P28329
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of CHAT and the recovery rates were calculated by comparing the measured value to the expected amount of CHAT in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-104 | 96 |
EDTA plasma(n=5) | 87-105 | 95 |
heparin plasma(n=5) | 91-103 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 96-103% | 86-101% | 88-100% |
EDTA plasma(n=5) | 85-97% | 82-100% | 82-100% |
heparin plasma(n=5) | 85-99% | 83-98% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only