- Product Name
- Human TPA(Tissue Polypeptide Antigen) ELISA Kit
- Catalogue No.
- EH3901
- Alias
- TPA ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- TPA ELISA Kit allows for the in vitro quantitative determination of TPA concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of TPA and the recovery rates were calculated by comparing the measured value to the expected amount of TPA in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 88-101 | 95 |
EDTA plasma(n=5) | 87-102 | 93 |
heparin plasma(n=5) | 86-99 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 92-104% | 85-99% | 87-101% |
EDTA plasma(n=5) | 85-101% | 91-100% | 84-91% |
heparin plasma(n=5) | 80-99% | 87-99% | 84-93% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only