- Product Name
- Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
- Catalogue No.
- EH3625
- Alias
- pNF-H ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- pNF-H ELISA Kit allows for the in vitro quantitative determination of pNF-H? concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P12036
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of pNF-H? and the recovery rates were calculated by comparing the measured value to the expected amount of pNF-H? in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-103 | 95 |
EDTA plasma(n=5) | 87-103 | 97 |
heparin plasma(n=5) | 85-100 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pNF-H? and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 94-101% | 91-103% |
EDTA plasma(n=5) | 83-101% | 88-101% | 83-99% |
heparin plasma(n=5) | 84-100% | 80-100% | 82-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only