- Product Name
- Human PKCβ1(Protein Kinase C Beta 1) ELISA Kit
- Catalogue No.
- EH3586
- Alias
- PKCβ1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PKCβ1 ELISA Kit allows for the in vitro quantitative determination of PKCβ1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- UniProt ID
- P05771
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PKCβ1 and the recovery rates were calculated by comparing the measured value to the expected amount of PKCβ1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-100 | 96 |
EDTA plasma(n=5) | 85-102 | 94 |
heparin plasma(n=5) | 86-97 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCβ1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-97% | 87-103% | 85-101% |
EDTA plasma(n=5) | 82-90% | 83-98% | 87-101% |
heparin plasma(n=5) | 87-93% | 81-96% | 80-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only