- Product Name
- Human IFN-α(Interferon Alpha) ELISA Kit
- Catalogue No.
- EH3252
- Alias
- IFN-α ELISA Kit, Interferon Alpha ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-105 | 95 |
EDTA plasma(n=5) | 85-104 | 95 |
heparin plasma(n=5) | 87-100 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-94% | 86-104% | 92-105% |
EDTA plasma(n=5) | 83-98% | 85-100% | 86-101% |
heparin plasma(n=5) | 80-95% | 81-96% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only