Products

Human IFN-α(Interferon Alpha) ELISA Kit

EH3252 Catalogue No.
IFN-α ELISA Kit, Interferon Alpha ELISA Kit
Reactivity:
Human
Range:
15.625-1000pg/ml
Sensitivity:
9.375pg/ml
Product Name
Human IFN-α(Interferon Alpha) ELISA Kit
Catalogue No.
EH3252
Alias
IFN-α ELISA Kit, Interferon Alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-10595
EDTA plasma(n=5)85-10495
heparin plasma(n=5)87-10094
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)85-94%86-104%92-105%
EDTA plasma(n=5)83-98%85-100%86-101%
heparin plasma(n=5)80-95%81-96%80-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only