- Product Name
- Human GUCA2A(Guanylate Cyclase Activator 2A) ELISA Kit
- Catalogue No.
- EH3205
- Alias
- GUCA2A ELISA Kit, Guanylate cyclase-activating protein I ELISA Kit, GCAP-I ELISA Kit, Guanylate cyclase-activating protein 1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- GUCA2A ELISA Kit allows for the in vitro quantitative determination of GUCA2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- UniProt ID
- Q02747
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GUCA2A and the recovery rates were calculated by comparing the measured value to the expected amount of GUCA2A in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 93-101 | 96 |
EDTA plasma(n=5) | 91-105 | 98 |
heparin plasma(n=5) | 85-101 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GUCA2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-97% | 97-103% | 85-103% |
EDTA plasma(n=5) | 85-96% | 83-95% | 87-96% |
heparin plasma(n=5) | 87-98% | 81-98% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only