- Product Name
- Human GnRH(Gonadotropin Releasing Hormone) ELISA Kit
- Catalogue No.
- EH3155
- Alias
- GnRH ELISA Kit, Gonadotropin Releasing Hormone ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- GnRH ELISA Kit allows for the in vitro quantitative determination of GnRH concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- UniProt ID
- P01148
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GnRH and the recovery rates were calculated by comparing the measured value to the expected amount of GnRH in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-103 | 97 |
EDTA plasma(n=5) | 88-102 | 97 |
heparin plasma(n=5) | 85-105 | 100 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GnRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 86-101% | 86-102% |
EDTA plasma(n=5) | 85-97% | 82-99% | 85-95% |
heparin plasma(n=5) | 80-91% | 87-94% | 81-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only