Products

Human f-Hb(Free Haemoglobin) ELISA Kit

EH3069 Catalogue No.
f-Hb ELISA Kit, Free Haemoglobin ELISA Kit
Reactivity:
Human
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Human f-Hb(Free Haemoglobin) ELISA Kit
Catalogue No.
EH3069
Alias
f-Hb ELISA Kit, Free Haemoglobin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
f-Hb ELISA Kit allows for the in vitro quantitative determination of f-Hb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of f-Hb and the recovery rates were calculated by comparing the measured value to the expected amount of f-Hb in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10393
EDTA plasma(n=5)85-10196
heparin plasma(n=5)91-10297
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of f-Hb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)86-104%86-103%85-95%
EDTA plasma(n=5)87-96%88-101%84-94%
heparin plasma(n=5)80-98%81-95%81-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only