Products

Human C3a(Complement Component 3a) ELISA Kit

EH2734 Catalogue No.
C3a ELISA Kit, C3a-desArg ELISA Kit, ASP ELISA Kit, Anaphylatoxin ELISA Kit, C3 ELISA Kit
Reactivity:
Human
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Human C3a(Complement Component 3a) ELISA Kit
Catalogue No.
EH2734
Alias
C3a ELISA Kit, C3a-desArg ELISA Kit, ASP ELISA Kit, Anaphylatoxin ELISA Kit, C3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C3a ELISA Kit allows for the in vitro quantitative determination of C3a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
P01024
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of C3a and the recovery rates were calculated by comparing the measured value to the expected amount of C3a in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9591
EDTA plasma(n=5)88-10196
heparin plasma(n=5)91-10396
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)94-105%88-98%89-105%
EDTA plasma(n=5)87-101%82-97%86-100%
heparin plasma(n=5)88-95%83-97%80-85%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only