- Product Name
- Human ADAMTS12(A Disintegrin And Metalloproteinase With Thrombospondin 12) ELISA Kit
- Catalogue No.
- EH2586
- Alias
- ADAMTS12 ELISA Kit, Human ADAMTS12 ELISA Kit, A Disintegrin And Metalloproteinase With Thrombospondin 12 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ADAMTS12 ELISA Kit allows for the in vitro quantitative determination of ADAMTS12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P58397
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ADAMTS12 and the recovery rates were calculated by comparing the measured value to the expected amount of ADAMTS12 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 88-102 | 97 |
EDTA plasma(n=5) | 85-102 | 92 |
heparin plasma(n=5) | 85-100 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADAMTS12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-105% | 86-105% | 87-105% |
EDTA plasma(n=5) | 90-99% | 86-101% | 82-100% |
heparin plasma(n=5) | 80-95% | 83-100% | 81-94% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only