- Product Name
- Human 14-3-3 protein eta(YWHAH) ELISA Kit
- Catalogue No.
- EH2534
- Alias
- YWHA1 ELISA Kit, Protein AS1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- 14-3-3 protein eta ELISA Kit allows for the in vitro quantitative determination of 14-3-3 protein eta concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.625-40ng/ml
- Sensitivity
- < 0.375ng/ml
- Species
- Human
- UniProt ID
- Q04917
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of 14-3-3 protein eta and the recovery rates were calculated by comparing the measured value to the expected amount of 14-3-3 protein eta in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-101 | 91 |
EDTA plasma(n=5) | 90-105 | 99 |
heparin plasma(n=5) | 89-98 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein eta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-103% | 90-97% | 86-105% |
EDTA plasma(n=5) | 83-95% | 83-101% | 85-100% |
heparin plasma(n=5) | 83-98% | 83-98% | 83-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only