- Product Name
- Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit
- Catalogue No.
- EH2006
- Alias
- GAPDH ELISA Kit, Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit, Peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit, GAPD ELISA Kit, G3PD ELISA Kit, G3PDH ELISA Kit, aging-associated gene 9 protein ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- GAPDH ELISA Kit allows for the in vitro quantitative determination of GAPDH concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- UniProt ID
- P04406
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GAPDH and the recovery rates were calculated by comparing the measured value to the expected amount of GAPDH in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 94-99 | 97 |
EDTA plasma(n=5) | 89-105 | 98 |
heparin plasma(n=5) | 89-104 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAPDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-101% | 87-97% | 92-102% |
EDTA plasma(n=5) | 82-97% | 86-101% | 85-98% |
heparin plasma(n=5) | 85-98% | 80-98% | 84-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only