Human PRKACA(cAMP-dependent protein kinase catalytic subunit alpha) ELISA Kit
EH1999
Catalogue No.
- PRKACA ELISA Kit, cAMP-dependent protein kinase catalytic subunit alpha ELISA Kit, PKA C-alpha ELISA Kit
- Reactivity:
- Human
- Range:
- 0.781-50ng/ml
- Sensitivity:
- 0.469ng/ml
- Product Name
- Human PRKACA(cAMP-dependent protein kinase catalytic subunit alpha) ELISA Kit
- Catalogue No.
- EH1999
- Alias
- PRKACA ELISA Kit, cAMP-dependent protein kinase catalytic subunit alpha ELISA Kit, PKA C-alpha ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PRKACA ELISA Kit allows for the in vitro quantitative determination of PRKACA concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Human
- UniProt ID
- P17612
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PRKACA and the recovery rates were calculated by comparing the measured value to the expected amount of PRKACA in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-103 95 EDTA plasma(n=5) 87-100 93 heparin plasma(n=5) 85-98 92 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRKACA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 87-99% 87-100% 88-103% EDTA plasma(n=5) 82-94% 94-99% 83-98% heparin plasma(n=5) 86-96% 83-100% 82-97% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only