- Product Name
- Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit
- Catalogue No.
- EH1980
- Alias
- 12-LO ELISA Kit, Arachidonate 12-Lipoxygenase ELISA Kit, ALOX12 ELISA Kit, LOG12 ELISA Kit, 12S-LOX ELISA Kit, 12S-lipoxygenase ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- 12-LO ELISA Kit allows for the in vitro quantitative determination of 12-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- P18054
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of 12-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 12-LO in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-105 | 97 |
EDTA plasma(n=5) | 86-101 | 94 |
heparin plasma(n=5) | 87-99 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-97% | 85-105% | 85-102% |
EDTA plasma(n=5) | 82-94% | 88-96% | 83-101% |
heparin plasma(n=5) | 80-99% | 81-96% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only