- Product Name
- Human EN2(Homeobox protein engrailed-2) ELISA Kit
- Catalogue No.
- EH1964
- Alias
- EN2 ELISA Kit, Homeobox protein engrailed-2 ELISA Kit, AUTS10 ELISA Kit, engrailed homeobox 2 ELISA Kit, engrailed homolog 2 ELISA Kit, engrailed-2 ELISA Kit, Homeobox protein en-2 ELISA Kit, hu-En-2 ELISA Kit, Hu-En-2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- EN2 ELISA Kit allows for the in vitro quantitative determination of EN2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- P19622
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of EN2 and the recovery rates were calculated by comparing the measured value to the expected amount of EN2 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-103 | 95 |
EDTA plasma(n=5) | 85-105 | 97 |
heparin plasma(n=5) | 89-105 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 85-101% | 88-104% |
EDTA plasma(n=5) | 82-100% | 85-101% | 83-91% |
heparin plasma(n=5) | 80-89% | 89-95% | 81-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only