Products

Human VCL(Vinculin) ELISA Kit

EH1957 Catalogue No.
VCL ELISA Kit, Vinculin ELISA Kit, MVCL ELISA Kit, CMD1W ELISA Kit, CMH15 ELISA Kit, Metavinculin ELISA Kit
Reactivity:
Human
Range:
78.125-5000pg/ml
Sensitivity:
46.875pg/ml
Product Name
Human VCL(Vinculin) ELISA Kit
Catalogue No.
EH1957
Alias
VCL ELISA Kit, Vinculin ELISA Kit, MVCL ELISA Kit, CMD1W ELISA Kit, CMH15 ELISA Kit, Metavinculin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VCL ELISA Kit allows for the in vitro quantitative determination of VCL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
P18206
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of VCL and the recovery rates were calculated by comparing the measured value to the expected amount of VCL in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)92-9996
EDTA plasma(n=5)86-10095
heparin plasma(n=5)85-9693
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-104%87-103%85-90%
EDTA plasma(n=5)87-100%90-99%82-99%
heparin plasma(n=5)84-96%81-99%86-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only