- Product Name
- Human VCL(Vinculin) ELISA Kit
- Catalogue No.
- EH1957
- Alias
- VCL ELISA Kit, Vinculin ELISA Kit, MVCL ELISA Kit, CMD1W ELISA Kit, CMH15 ELISA Kit, Metavinculin ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- VCL ELISA Kit allows for the in vitro quantitative determination of VCL concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P18206
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of VCL and the recovery rates were calculated by comparing the measured value to the expected amount of VCL in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 92-99 | 96 |
EDTA plasma(n=5) | 86-100 | 95 |
heparin plasma(n=5) | 85-96 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-104% | 87-103% | 85-90% |
EDTA plasma(n=5) | 87-100% | 90-99% | 82-99% |
heparin plasma(n=5) | 84-96% | 81-99% | 86-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only