Products

Human SFN(14-3-3 protein sigma) ELISA Kit

EH1771 Catalogue No.
SFN ELISA Kit, 14-3-3 protein sigma ELISA Kit, Epithelial cell marker protein 1 ELISA Kit, StRatifin ELISA Kit, HME1 ELISA Kit, YWHAS ELISA Kit
Reactivity:
Human
Range:
78.125-5000pg/ml
Sensitivity:
46.875pg/ml
Product Name
Human SFN(14-3-3 protein sigma) ELISA Kit
Catalogue No.
EH1771
Alias
SFN ELISA Kit, 14-3-3 protein sigma ELISA Kit, Epithelial cell marker protein 1 ELISA Kit, StRatifin ELISA Kit, HME1 ELISA Kit, YWHAS ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SFN ELISA Kit allows for the in vitro quantitative determination of SFN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
P31947
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of SFN and the recovery rates were calculated by comparing the measured value to the expected amount of SFN in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10598
EDTA plasma(n=5)90-10496
heparin plasma(n=5)85-10093
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SFN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)96-104%89-104%97-105%
EDTA plasma(n=5)91-99%82-94%85-89%
heparin plasma(n=5)80-89%89-100%82-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only