- Product Name
- Human SFN(14-3-3 protein sigma) ELISA Kit
- Catalogue No.
- EH1771
- Alias
- SFN ELISA Kit, 14-3-3 protein sigma ELISA Kit, Epithelial cell marker protein 1 ELISA Kit, StRatifin ELISA Kit, HME1 ELISA Kit, YWHAS ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- SFN ELISA Kit allows for the in vitro quantitative determination of SFN concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P31947
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of SFN and the recovery rates were calculated by comparing the measured value to the expected amount of SFN in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-105 | 98 |
EDTA plasma(n=5) | 90-104 | 96 |
heparin plasma(n=5) | 85-100 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SFN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 96-104% | 89-104% | 97-105% |
EDTA plasma(n=5) | 91-99% | 82-94% | 85-89% |
heparin plasma(n=5) | 80-89% | 89-100% | 82-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only