- Product Name
- Human AIF1(Allograft inflammatory factor 1) ELISA Kit
- Catalogue No.
- EH1425
- Alias
- AIF1 ELISA Kit, Allograft Inflammatory Factor 1 ELISA Kit, IBA1 ELISA Kit, IRT1 ELISA Kit, ionized calcium-binding adapter molecule 1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- AIF1 ELISA Kit allows for the in vitro quantitative determination of AIF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- UniProt ID
- P55008
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of AIF1 and the recovery rates were calculated by comparing the measured value to the expected amount of AIF1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-105 | 93 |
EDTA plasma(n=5) | 85-102 | 93 |
heparin plasma(n=5) | 85-97 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AIF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-104% | 89-104% | 87-101% |
EDTA plasma(n=5) | 85-100% | 84-98% | 82-99% |
heparin plasma(n=5) | 80-98% | 87-98% | 80-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only