- Product Name
- Human ALPP(Alkaline phosphatase, placental type) ELISA Kit
- Catalogue No.
- EH1391
- Alias
- PLAP ELISA Kit, ALPP ELISA Kit, Placental Alkaline Phosphatase ELISA Kit, Alkaline phosphatase Regan isozyme ELISA Kit, Placental alkaline phosphatase 1 ELISA Kit, PLAP-1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ALPP ELISA Kit allows for the in vitro quantitative determination of ALPP concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- UniProt ID
- P05187
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ALPP and the recovery rates were calculated by comparing the measured value to the expected amount of ALPP in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-105 | 95 |
EDTA plasma(n=5) | 87-102 | 93 |
heparin plasma(n=5) | 87-103 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALPP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-100% | 86-99% | 89-101% |
EDTA plasma(n=5) | 87-100% | 83-96% | 85-98% |
heparin plasma(n=5) | 80-98% | 85-95% | 80-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only