- Product Name
- Human VAPB(Vesicle-associated membrane protein-associated protein B/C) ELISA Kit
- Catalogue No.
- EH13540
- Alias
- Vesicle-associated membrane protein-associated protein B/C ELISA Kit, VAMP-B/VAMP-C ELISA Kit, VAMP-associated protein B/C ELISA Kit, VAP-B/VAP-C ELISA Kit, UNQ484 ELISA Kit, PRO983 ELISA Kit, VAPB ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- VAPB ELISA Kit allows for the in vitro quantitative determination of VAPB concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- UniProt ID
- O95292
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of VAPB and the recovery rates were calculated by comparing the measured value to the expected amount of VAPB in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-96 | 91 |
EDTA plasma(n=5) | 87-99 | 93 |
heparin plasma(n=5) | 88-105 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VAPB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 92-96% | 86-103% | 86-101% |
EDTA plasma(n=5) | 86-95% | 87-101% | 85-95% |
heparin plasma(n=5) | 80-100% | 85-98% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only