- Product Name
- Human PDE10A (cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A) ELISA Kit
- Catalogue No.
- EH1324
- Alias
- PDE10A ELISA Kit, cAMP and cAMP-inhibited cGMP 3' ELISA Kit, 5'-cyclic phosphodiesterase 10A ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PDE10A ELISA Kit allows for the in vitro quantitative determination of PDE10A concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- Q9Y233
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PDE10A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE10A in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-96 | 93 |
EDTA plasma(n=5) | 88-105 | 96 |
heparin plasma(n=5) | 85-104 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE10A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-102% | 88-105% | 86-95% |
EDTA plasma(n=5) | 87-99% | 87-99% | 85-98% |
heparin plasma(n=5) | 85-98% | 91-97% | 85-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only