- Product Name
- Human RAB27B(Ras-related protein Rab-27B) ELISA Kit
- Catalogue No.
- EH11587
- Alias
- C25KG ELISA Kit, RAB27B ELISA Kit, Ras related protein Rab 27B ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- RAB27B ELISA Kit allows for the in vitro quantitative determination of RAB27B concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- O00194
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of RAB27B and the recovery rates were calculated by comparing the measured value to the expected amount of RAB27B in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-104 | 96 |
EDTA plasma(n=5) | 85-97 | 91 |
heparin plasma(n=5) | 94-100 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAB27B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-99% | 89-98% | 85-100% |
EDTA plasma(n=5) | 89-101% | 82-97% | 86-101% |
heparin plasma(n=5) | 81-99% | 81-100% | 85-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only