- Product Name
- Human CYB5R2(NADH-cytochrome b5 reductase 2) ELISA Kit
- Catalogue No.
- EH1135
- Alias
- CYB5R2 ELISA Kit, NADH-cytochrome b5 reductase 2 ELISA Kit, b52 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CYB5R2 ELISA Kit allows for the in vitro quantitative determination of CYB5R2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- Q6BCY4
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of CYB5R2 and the recovery rates were calculated by comparing the measured value to the expected amount of CYB5R2 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-95 | 91 |
EDTA plasma(n=5) | 85-97 | 93 |
heparin plasma(n=5) | 87-103 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYB5R2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-102% | 92-105% | 86-103% |
EDTA plasma(n=5) | 83-101% | 83-99% | 82-100% |
heparin plasma(n=5) | 88-98% | 85-99% | 80-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only