Human PDE4A (cAMP-specific 3′,5′-cyclic phosphodiesterase 4A) ELISA Kit
EH10975
Catalogue No.
- cAMP-specific 3',5'-cyclic phosphodiesterase 4A ELISA Kit, DPDE2 ELISA Kit, PDE46 ELISA Kit, PDE4A ELISA Kit
- Reactivity:
- Human
- Range:
- 31.25-2000pg/ml
- Sensitivity:
- 18.75pg/ml
- Product Name
- Human PDE4A (cAMP-specific 3′,5′-cyclic phosphodiesterase 4A) ELISA Kit
- Catalogue No.
- EH10975
- Alias
- cAMP-specific 3',5'-cyclic phosphodiesterase 4A ELISA Kit, DPDE2 ELISA Kit, PDE46 ELISA Kit, PDE4A ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PDE4A ELISA Kit allows for the in vitro quantitative determination of PDE4A concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- UniProt ID
- P27815
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PDE4A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4A in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 87-104 94 EDTA plasma(n=5) 88-101 93 heparin plasma(n=5) 88-94 91 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 88-104% 89-102% 87-104% EDTA plasma(n=5) 85-100% 84-96% 84-101% heparin plasma(n=5) 83-92% 86-100% 85-92% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only