- Product Name
- Human MRPL53(Mitochondrial large ribosomal subunit protein mL53) ELISA Kit
- Catalogue No.
- EH10236
- Alias
- 39S ribosomal protein L53 ELISA Kit, mitochondrial ELISA Kit, L53mt ELISA Kit, MRP-L53 ELISA Kit, Mitochondrial large ribosomal subunit protein mL53 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- MRPL53 ELISA Kit allows for the in vitro quantitative determination of MRPL53 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- UniProt ID
- Q96EL3
- Standard curve
- Storage
- 2-8 °C for 6 months
- Recovery
Matrices listed below were spiked with certain level of MRPL53 and the recovery rates were calculated by comparing the measured value to the expected amount of MRPL53 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-105 | 96 |
EDTA plasma(n=5) | 86-105 | 96 |
heparin plasma(n=5) | 86-101 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MRPL53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-103% | 85-105% | 92-102% |
EDTA plasma(n=5) | 85-101% | 85-98% | 82-96% |
heparin plasma(n=5) | 84-100% | 84-100% | 81-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only