- Product Name
- Human F12(Coagulation factor XII) ELISA Kit
- Catalogue No.
- EH1012
- Alias
- F12 ELISA Kit, Coagulation factor XII ELISA Kit, HAEX ELISA Kit, Hageman factor ELISA Kit, HAE3 ELISA Kit, HAF ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- F12 ELISA Kit allows for the in vitro quantitative determination of F12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 3.125-200ng/ml
- Sensitivity
- < 1.875ng/ml
- Species
- Human
- UniProt ID
- P00748
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of F12 and the recovery rates were calculated by comparing the measured value to the expected amount of F12 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-100 | 95 |
EDTA plasma(n=5) | 89-105 | 99 |
heparin plasma(n=5) | 86-98 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-95% | 87-101% | 85-102% |
EDTA plasma(n=5) | 86-99% | 82-92% | 93-101% |
heparin plasma(n=5) | 82-99% | 83-99% | 81-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only