- Product Name
- Human GPT(Alanine aminotransferase 1) ELISA Kit
- Catalogue No.
- EH0770
- Alias
- GPT ELISA Kit, ALT1 ELISA Kit, Alanine aminotransferase 1 ELISA Kit, GPT 1 ELISA Kit, AAT1 ELISA Kit, Glutamic--pyruvic transaminase 1 ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- GPT ELISA Kit allows for the in vitro quantitative determination of GPT concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 3.125-200mIU/ml
- Sensitivity
- < 1.875mIU/ml
- Species
- Human
- UniProt ID
- P24298
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GPT and the recovery rates were calculated by comparing the measured value to the expected amount of GPT in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-102 | 94 |
EDTA plasma(n=5) | 94-101 | 98 |
heparin plasma(n=5) | 92-103 | 99 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-103% | 88-102% | 89-97% |
EDTA plasma(n=5) | 83-100% | 82-101% | 83-95% |
heparin plasma(n=5) | 81-100% | 80-92% | 84-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only