- Product Name
- Human CD28(T-cell-specific surface glycoprotein CD28) ELISA Kit
- Catalogue No.
- EH0653
- Alias
- CD28 ELISA Kit, CD28 antigen ELISA Kit, CD28 antigen ELISA Kit, Tp44 ELISA Kit, CD28 molecule ELISA Kit, TP44 ELISA Kit, Tp44 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CD28 ELISA Kit allows for the in vitro quantitative determination of CD28 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.625-40ng/ml
- Sensitivity
- < 0.375ng/ml
- Species
- Human
- UniProt ID
- P10747
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of CD28 and the recovery rates were calculated by comparing the measured value to the expected amount of CD28 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-104 | 96 |
EDTA plasma(n=5) | 85-100 | 93 |
heparin plasma(n=5) | 88-105 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD28 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-104% | 86-101% | 89-104% |
EDTA plasma(n=5) | 85-99% | 83-100% | 85-92% |
heparin plasma(n=5) | 85-97% | 83-95% | 86-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only