- Product Name
- Human STAT3(Signal transducer and activator of transcription 3) ELISA Kit
- Catalogue No.
- EH0602
- Alias
- STAT3 ELISA Kit, Signal Transducer and Activator of Transcription 3 ELISA Kit, Acute-phase response factor ELISA Kit, APRFMGC16063 ELISA Kit, DNA-binding protein APRF ELISA Kit, HIES ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- STAT3 ELISA Kit allows for the in vitro quantitative determination of STAT3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- P40763
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of STAT3 and the recovery rates were calculated by comparing the measured value to the expected amount of STAT3 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-100 | 92 |
EDTA plasma(n=5) | 85-97 | 92 |
heparin plasma(n=5) | 88-105 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STAT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-101% | 87-96% | 87-93% |
EDTA plasma(n=5) | 87-99% | 83-96% | 83-101% |
heparin plasma(n=5) | 82-96% | 81-96% | 80-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only