Products

Human 5-NT(5-Nucleotidase) ELISA Kit

EH0468 Catalogue No.
5-NT ELISA Kit, NT5E ELISA Kit, 5'-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5' nucleotidase ELISA Kit, CD73 ELISA Kit, CD73 antigen ELISA Kit, Ecto-5'-nucleotidase ELISA Kit, 5'-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit
Reactivity:
Human
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Human 5-NT(5-Nucleotidase) ELISA Kit
Catalogue No.
EH0468
Alias
5-NT ELISA Kit, NT5E ELISA Kit, 5'-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5' nucleotidase ELISA Kit, CD73 ELISA Kit, CD73 antigen ELISA Kit, Ecto-5'-nucleotidase ELISA Kit, 5'-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
P21589
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of 5-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-NT in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)90-10397
EDTA plasma(n=5)91-10498
heparin plasma(n=5)86-10193
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)88-103%94-104%89-96%
EDTA plasma(n=5)87-96%82-101%82-99%
heparin plasma(n=5)82-100%80-97%80-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only