- Product Name
- Human 5-NT(5-Nucleotidase) ELISA Kit
- Catalogue No.
- EH0468
- Alias
- 5-NT ELISA Kit, NT5E ELISA Kit, 5'-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5' nucleotidase ELISA Kit, CD73 ELISA Kit, CD73 antigen ELISA Kit, Ecto-5'-nucleotidase ELISA Kit, 5'-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- 5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- P21589
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of 5-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-NT in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-103 | 97 |
EDTA plasma(n=5) | 91-104 | 98 |
heparin plasma(n=5) | 86-101 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-103% | 94-104% | 89-96% |
EDTA plasma(n=5) | 87-96% | 82-101% | 82-99% |
heparin plasma(n=5) | 82-100% | 80-97% | 80-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only