- Product Name
- Human PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit
- Catalogue No.
- EH0254
- Alias
- PDGF-BB ELISA Kit, Platelet Derived Growth Factor-BB ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PDGF-BB ELISA Kit allows for the in vitro quantitative determination of PDGF-BB concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PDGF-BB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGF-BB in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-101 | 93 |
EDTA plasma(n=5) | 90-104 | 97 |
heparin plasma(n=5) | 87-104 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGF-BB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-102% | 89-100% | 86-104% |
EDTA plasma(n=5) | 87-101% | 82-101% | 83-100% |
heparin plasma(n=5) | 81-88% | 81-99% | 83-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only