Human OPN(Osteopontin) ELISA Kit

EH0248 Catalogue No.

Alias:: OPN ELISA Kit, Osteopontin ELISA Kit, Eta-1 ELISA Kit, Spp1 ELISA Kit, BNSP ELISA Kit, Bone sialoprotein 1 ELISA Kit, MGC110940 ELISA Kit, Nephropontin ELISA Kit, secreted phosphoprotein-1 ELISA Kit, osteopontin ELISA Kit, bone sialoprotein ELISA Kit, SPP-1 ELISA Kit, SPP1 ELISA Kit, CALPHA1 fusion ELISA Kit, Urinary stone protein ELISA Kit, uropontin ELISA Kit
Reactivity:: Human
Range:: 0.156-10ng/ml
Sensitivity:: 0.094ng/ml

Manual

Specifications
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FAQs

Product Name

Human OPN(Osteopontin) ELISA Kit

Catalogue No.

EH0248

Alias

OPN ELISA Kit, Osteopontin ELISA Kit, Eta-1 ELISA Kit, Spp1 ELISA Kit, BNSP ELISA Kit, Bone sialoprotein 1 ELISA Kit, MGC110940 ELISA Kit, Nephropontin ELISA Kit, secreted phosphoprotein-1 ELISA Kit, osteopontin ELISA Kit, bone sialoprotein ELISA Kit, SPP-1 ELISA Kit, SPP1 ELISA Kit, CALPHA1 fusion ELISA Kit, Urinary stone protein ELISA Kit, uropontin ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

OPN ELISA Kit allows for the in vitro quantitative determination of OPN concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P10451

Standard curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of OPN and the recovery rates were calculated by comparing the measured value to the expected amount of OPN in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-101	92
EDTA plasma(n=5)	85-100	90
heparin plasma(n=5)	86-102	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.