- Product Name
- Human MIA(Melanoma Inhibitory Activity Protein 1) ELISA Kit
- Catalogue No.
- EH0227
- Alias
- MIA1 ELISA Kit, MIA ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- MIA ELISA Kit allows for the in vitro quantitative determination of MIA concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 7.813-500pg/ml
- Sensitivity
- < 4.688pg/ml
- Species
- Human
- UniProt ID
- Q16674
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of MIA and the recovery rates were calculated by comparing the measured value to the expected amount of MIA in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-101 | 93 |
EDTA plasma(n=5) | 85-99 | 93 |
heparin plasma(n=5) | 87-100 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-100% | 85-102% | 87-102% |
EDTA plasma(n=5) | 86-99% | 82-101% | 82-97% |
heparin plasma(n=5) | 81-99% | 87-99% | 80-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only