Human LEP(Leptin) ELISA Kit 
EH0216
Catalogue No.
- Leptin ELISA Kit, LEP ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit
- Reactivity:
- Human
- Range:
- 31.25-2000pg/ml
- Sensitivity:
- 18.75pg/ml
- Product Name
- Human LEP(Leptin) ELISA Kit
- Catalogue No.
- EH0216
- Alias
- Leptin ELISA Kit, LEP ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- LEP ELISA Kit allows for the in vitro quantitative determination of LEP concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Human
- UniProt ID
- P41159
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of LEP and the recovery rates were calculated by comparing the measured value to the expected amount of LEP in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-99 92 EDTA plasma(n=5) 93-101 97 heparin plasma(n=5) 87-105 94 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 90-103% 99-102% 90-103% EDTA plasma(n=5) 86-97% 86-100% 84-97% heparin plasma(n=5) 82-90% 80-100% 90-100% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only
