- Product Name
- Human IL-12(Interleukin 12) ELISA Kit
- Catalogue No.
- EH0176
- Alias
- Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-104 | 91 |
EDTA plasma(n=5) | 91-103 | 100 |
heparin plasma(n=5) | 88-101 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 98-105% | 92-99% | 88-99% |
EDTA plasma(n=5) | 85-96% | 84-101% | 82-95% |
heparin plasma(n=5) | 86-94% | 85-96% | 80-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only