Products

Human IL-12(Interleukin 12) ELISA Kit

EH0176 Catalogue No.
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit
Reactivity:
Human
Range:
15.625-1000pg/ml
Sensitivity:
9.375pg/ml
Product Name
Human IL-12(Interleukin 12) ELISA Kit
Catalogue No.
EH0176
Alias
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10491
EDTA plasma(n=5)91-103100
heparin plasma(n=5)88-10193
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)98-105%92-99%88-99%
EDTA plasma(n=5)85-96%84-101%82-95%
heparin plasma(n=5)86-94%85-96%80-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only