- Product Name
- Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit
- Catalogue No.
- EH0160
- Alias
- HSP27 ELISA Kit, Heat Shock Protein 27 ELISA Kit, HSP-27 ELISA Kit, HSPB1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- HSP27/HSPB1 ELISA Kit allows for the in vitro quantitative determination of HSP27/HSPB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Human
- UniProt ID
- P04792
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of HSP27/HSPB1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP27/HSPB1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-104 | 98 |
EDTA plasma(n=5) | 85-101 | 94 |
heparin plasma(n=5) | 85-105 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP27/HSPB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-105% | 88-93% | 88-102% |
EDTA plasma(n=5) | 84-97% | 83-98% | 93-101% |
heparin plasma(n=5) | 86-94% | 81-91% | 85-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only