Products

Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit

EH0160 Catalogue No.
HSP27 ELISA Kit, Heat Shock Protein 27 ELISA Kit, HSP-27 ELISA Kit, HSPB1 ELISA Kit
Reactivity:
Human
Range:
0.781-50ng/ml
Sensitivity:
0.469ng/ml
Product Name
Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit
Catalogue No.
EH0160
Alias
HSP27 ELISA Kit, Heat Shock Protein 27 ELISA Kit, HSP-27 ELISA Kit, HSPB1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HSP27/HSPB1 ELISA Kit allows for the in vitro quantitative determination of HSP27/HSPB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
P04792
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of HSP27/HSPB1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP27/HSPB1 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10498
EDTA plasma(n=5)85-10194
heparin plasma(n=5)85-10595
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP27/HSPB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)86-105%88-93%88-102%
EDTA plasma(n=5)84-97%83-98%93-101%
heparin plasma(n=5)86-94%81-91%85-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only