- Product Name
- Human G-CSF(Granulocyte Colony Stimulating Factor 3) ELISA Kit
- Catalogue No.
- EH0149
- Alias
- G-CSF ELISA Kit, CSF3 ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, Pluripoietin ELISA Kit, Filgrastim ELISA Kit, Lenograstim ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- G-CSF ELISA Kit allows for the in vitro quantitative determination of G-CSF concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 39.063-2500pg/ml
- Sensitivity
- < 23.438pg/ml
- Species
- Human
- UniProt ID
- P09919
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of G-CSF and the recovery rates were calculated by comparing the measured value to the expected amount of G-CSF in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-103 | 96 |
EDTA plasma(n=5) | 86-104 | 94 |
heparin plasma(n=5) | 90-100 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of G-CSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-91% | 88-99% | 86-105% |
EDTA plasma(n=5) | 85-99% | 90-97% | 88-96% |
heparin plasma(n=5) | 84-90% | 87-94% | 82-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only