Human C5a(Complement Component 5a) ELISA Kit 
EH0096
Catalogue No.
- C5a ELISA Kit, Complement Component 5a ELISA Kit
- Reactivity:
- Human
- Range:
- 78.125-5000pg/ml
- Sensitivity:
- 46.875pg/ml
- Product Name
- Human C5a(Complement Component 5a) ELISA Kit
- Catalogue No.
- EH0096
- Alias
- C5a ELISA Kit, Complement Component 5a ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- C5a ELISA Kit allows for the in vitro quantitative determination of C5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P01031
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of C5a and the recovery rates were calculated by comparing the measured value to the expected amount of C5a in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 89-105 96 EDTA plasma(n=5) 89-105 96 heparin plasma(n=5) 88-103 95 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 85-105% 85-100% 85-102% EDTA plasma(n=5) 83-101% 83-101% 84-98% heparin plasma(n=5) 81-97% 83-95% 83-97% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only
