- Product Name
- Human MIF(Macrophage migRation inhibitory factor) ELISA Kit
- Catalogue No.
- EH0016
- Alias
- MIF ELISA Kit, Macrophage migRation inhibitory factor ELISA Kit, GIFmacrophage migRation inhibitory factor ELISA Kit, GLIF ELISA Kit, Glycosylation-inhibiting factor ELISA Kit, L-dopachrome isomerase ELISA Kit, L-dopachrome tautomerase ELISA Kit, macrophage migRation inhibitory factor ELISA Kit, glycosylation-inhibiting factor ELISA Kit, MMIF ELISA Kit, Phenylpyruvate tautomerase ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- MIF ELISA Kit allows for the in vitro quantitative determination of MIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Human
- UniProt ID
- P14174
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of MIF and the recovery rates were calculated by comparing the measured value to the expected amount of MIF in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-98 | 92 |
EDTA plasma(n=5) | 93-101 | 97 |
heparin plasma(n=5) | 86-105 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-104% | 85-100% | 90-104% |
EDTA plasma(n=5) | 82-93% | 92-101% | 86-94% |
heparin plasma(n=5) | 82-96% | 80-97% | 85-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only