Products

Human MIF(Macrophage migRation inhibitory factor) ELISA Kit

EH0016 Catalogue No.
MIF ELISA Kit, Macrophage migRation inhibitory factor ELISA Kit, GIFmacrophage migRation inhibitory factor ELISA Kit, GLIF ELISA Kit, Glycosylation-inhibiting factor ELISA Kit, L-dopachrome isomerase ELISA Kit, L-dopachrome tautomerase ELISA Kit, macrophage migRation inhibitory factor ELISA Kit, glycosylation-inhibiting factor ELISA Kit, MMIF ELISA Kit, Phenylpyruvate tautomerase ELISA Kit
Reactivity:
Human
Range:
0.781-50ng/ml
Sensitivity:
0.469ng/ml
Product Name
Human MIF(Macrophage migRation inhibitory factor) ELISA Kit
Catalogue No.
EH0016
Alias
MIF ELISA Kit, Macrophage migRation inhibitory factor ELISA Kit, GIFmacrophage migRation inhibitory factor ELISA Kit, GLIF ELISA Kit, Glycosylation-inhibiting factor ELISA Kit, L-dopachrome isomerase ELISA Kit, L-dopachrome tautomerase ELISA Kit, macrophage migRation inhibitory factor ELISA Kit, glycosylation-inhibiting factor ELISA Kit, MMIF ELISA Kit, Phenylpyruvate tautomerase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MIF ELISA Kit allows for the in vitro quantitative determination of MIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
P14174
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of MIF and the recovery rates were calculated by comparing the measured value to the expected amount of MIF in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-9892
EDTA plasma(n=5)93-10197
heparin plasma(n=5)86-10595
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-104%85-100%90-104%
EDTA plasma(n=5)82-93%92-101%86-94%
heparin plasma(n=5)82-96%80-97%85-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only