- Product Name
- Chicken CNTF(Ciliary Neurotrophic Factor) ELISA Kit
- Catalogue No.
- ECH0015
- Alias
- CNTF ELISA Kit, HCNTF ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CNTF ELISA Kit allows for the in vitro quantitative determination of CNTF concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 62.5-4000pg/ml
- Sensitivity
- < 37.5pg/ml
- Species
- Chicken
- UniProt ID
- Q02011
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of CNTF and the recovery rates were calculated by comparing the measured value to the expected amount of CNTF in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-102 | 93 |
EDTA plasma(n=5) | 87-97 | 92 |
heparin plasma(n=5) | 86-101 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CNTF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 91-98% | 85-98% | 90-103% |
EDTA plasma(n=5) | 86-99% | 84-96% | 84-95% |
heparin plasma(n=5) | 82-97% | 88-97% | 81-94% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only