- Product Name
- Bovine E2 (Estradiol) ELISA Kit
- Catalogue No.
- EB0081
- Alias
- E2 ELISA Kit, Estradiol ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- E2 ELISA Kit allows for the in vitro quantitative determination of E2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 12.5-800pg/ml
- Sensitivity
- < 7.5pg/ml
- Species
- Bovine
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-104 | 94 |
EDTA plasma(n=5) | 89-98 | 91 |
heparin plasma(n=5) | 88-102 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-98% | 88-99% | 86-95% |
EDTA plasma(n=5) | 82-93% | 83-101% | 93-100% |
heparin plasma(n=5) | 80-93% | 82-92% | 80-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only