- Product Name
- Bovine HSP60(Heat shock protein 60) ELISA Kit
- Catalogue No.
- EB0031
- Alias
- Heat shock protein 60 ELISA Kit, HSP 60 ELISA Kit, HSP60 ELISA Kit, HSP65 ELISA Kit, HSPD1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- HSP60 ELISA Kit allows for the in vitro quantitative determination of HSP60 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563 -100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Bovine
- UniProt ID
- P31081
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of HSP60 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP60 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-100 | 94 |
EDTA plasma(n=5) | 86-105 | 95 |
heparin plasma(n=5) | 86-103 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP60 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-104% | 86-105% | 86-101% |
EDTA plasma(n=5) | 83-101% | 82-90% | 84-101% |
heparin plasma(n=5) | 85-99% | 82-98% | 87-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only