- Product Name
- Bovine P21(Cyclin-dependent Kinase Inhibitor 1A) ELISA Kit
- Catalogue No.
- EB0014
- Alias
- CDKN1A ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- P21 ELISA Kit allows for the in vitro quantitative determination of P21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Bovine
- UniProt ID
- A5PKM2
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of P21 and the recovery rates were calculated by comparing the measured value to the expected amount of P21 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-102 | 98 |
EDTA plasma(n=5) | 90-102 | 95 |
heparin plasma(n=5) | 91-98 | 96 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 90-102% | 85-105% | 89-102% |
EDTA plasma(n=5) | 83-98% | 88-101% | 89-101% |
heparin plasma(n=5) | 83-100% | 89-96% | 80-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only