Products

Bovine P21(Cyclin-dependent Kinase Inhibitor 1A) ELISA Kit

EB0014 Catalogue No.
CDKN1A ELISA Kit
Reactivity:
Bovine
Range:
78.125-5000pg/ml
Sensitivity:
46.875pg/ml
Product Name
Bovine P21(Cyclin-dependent Kinase Inhibitor 1A) ELISA Kit
Catalogue No.
EB0014
Alias
CDKN1A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
P21 ELISA Kit allows for the in vitro quantitative determination of P21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Bovine
UniProt ID
A5PKM2
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of P21 and the recovery rates were calculated by comparing the measured value to the expected amount of P21 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)90-10298
EDTA plasma(n=5)90-10295
heparin plasma(n=5)91-9896
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)90-102%85-105%89-102%
EDTA plasma(n=5)83-98%88-101%89-101%
heparin plasma(n=5)83-100%89-96%80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only