- Product Name
- Bovine Ig(Total Immunoglobulin) ELISA Kit
- Catalogue No.
- EB0001
- Alias
- Immunoglobulin ELISA Kit
- Detection Method
- HRP Competitive ELISA, Coated with Antigen
- Application
- Ig ELISA Kit allows for the in vitro quantitative determination of Ig concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.234-15ug/ml
- Sensitivity
- < 0.141ug/ml
- Species
- Bovine
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Ig and the recovery rates were calculated by comparing the measured value to the expected amount of Ig in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-99 | 93 |
EDTA plasma(n=5) | 86-105 | 96 |
heparin plasma(n=5) | 89-105 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ig and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 90-103% | 89-101% | 86-98% |
EDTA plasma(n=5) | 85-100% | 87-100% | 89-100% |
heparin plasma(n=5) | 88-94% | 88-98% | 87-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only